A long term evaluation of the UCX® cardioprotective effect after MI induction in vivo has revealed an even more drastic functional improvemnt of the performance of the ischemic myocardium when compared to the controls. A robust microultrasound system, with higher spatial and temporal resolution, contributed to increase the analysis detail and therefore to detect significant differences in functional parameters which were not clearly observed in the prior study. This experiment was also a step forward in the validation of UCX® efficacy, when delivered as an ATMP and, therefore, safer for clinical use. As before, this work was performed in straight colaboration with INEB, Porto and it will be presented in detail for the first time at the annual meeting of the Portuguese Society for Stem Cells and Cell Therapies, at ITQB/iBET, Oeiras, PT, to be held in October 15th and 16th, 2015.